Rafal Nazarewicz Spring Energy Founder and Manager

Spring Energy founder

Ultramarathon runner, nutrition specialist, cell and mitochondrial biologist experienced in human nutrition and bioenergetics, cell and mitochondria metabolism, molecular and cellular biology, research of oxidative stress, cell signaling, and exercise physiology

 

EDUCATION                                                     

  • Master degree, physical education; human nutrition
  • Ph. D., human nutrition;
 
  • Habilitation, Public health, Medical University of Gdansk

 

ACADEMIC APPOINTMENTS                                 

  • 2014-2016 Division of Immunology and Rheumatology , Stanford University School of Medicine, Stanford, CA
  • 2011-2016 Division of Clinical Pharmacology, and Free Radicals in Biology and Medicine Core, Vanderbilt University Medical Center, Nashville, TN 
  • 2007-2011  Emory University, School of Medicine, Cardiology Division, Atlanta, GA 
  • 2006-2007  The Ohio State University, Institute of Mitochondrial Biology, Davis Heart & Lung Research Institute, Columbus, OH
  • 2002-2006  J. Sniadecki University School of Physical Education, Department of Biochemistry, Poland
  • 2003-2006 Gdynia Maritime University, Division of Food and Nutrition Assessment, Poland
  • 1997-2002 Gdynia Maritime University, Division of Food and Nutrition, Poland
  • 1995-1997 J. Sniadecki University School of Physical Education, Department of Biochemistry, Poland

TRAINING: 

  • Postdoctoral training research training
  • 2007-2010 Emory University, School of Medicine, Division of Cardiology; Atlanta, GA 
  • 2006-2007 Ohio State University, Institute of Mitochondrial Biology, Davis Heart & Lung Research Institute; Columbus, OH 
  • Undergraduate research training
  • 1995-1997 Undergraduate research training; University of Physical Education and Sport, Department of Biochemistry and Bioenergetics, Poland

RESEARCH PROGRAM AWARDS

  • 2014-2018  “T-cell mitochondrial ROS in development of hypertension.” American Heart Association; Principal investigator  
  • 2010-2014 “Mitochondrial Oxidative Stress in Angiotensin II-induced Endothelial Dysfunction” NIH; Co-investigator
  • Gdynia Maritime University, Poland; travel grant to the Ohio State University
  • 2004-2006 “Effects of diet on oxidative stress in human subjects of various physical activity”, The State Committee for Scientific Research, Poland, Principal Investigator
  • 2002-2003 “Metabolic consequences of diet in young sportsmen”, The State Committee for Scientific Research, Poland; Co-investigator 

EXTRAMURAL ACTIVITIES

Ad hoc reviewer Circulation Research, Antioxidants and Redox Signaling, The International Journal of Biochemistry & Cell Biology


PROFESSIONAL ORGANIZATIONS

American Heart Association, American Physiological Society  


PUBLICATIONS
(*-corresponding author)

  1. Anna Dikalova, Vladimir Mayorov, Liang Xiao, Alexander Panov, Venkataraman Amarnath, Irene Zagol-Ikapitte, Aurelia Vergeade, Mingfang Ao, Valery Yermalitsky, Rafal Nazarewicz, Olivier Boutaud, Marcos Lopez, Frederic Billings IV, Sean Davies, L. Jackson Roberts, II, David Harrison, and Sergey Dikalov Mitochondrial Isolevuglandins Contribute to Vascular Oxidative Stress and Mitochondria-Targeted Scavenger of Isolevuglandins Reduces Mitochondrial Dysfunction and Hypertension. Hypertension 2020 Aug
  2. Dikalova AE, Itani HA, Nazarewicz RR, McMaster WG, Flynn CR, Uzhachenko RV, Fessel JP, Gamboa JL, Harrison DG, Dikalov SI. Sirt3 Impairment and SOD2 Hyperacetylation in Vascular Oxidative Stress and Hypertension. Circ Res. 2017 Jul 
  3. Shirai T, Nazarewicz RR, Tian L, Harrison DG, Wallis BB, Goronzy JJ, Weyand CM. PKM2 is a redox-sensitive regulator of proinflammatory macrophages effector functions in coronary artery disease. J Exp. Med. 2016 Jan.
  4. Itani HA, Dikalova AE, McMaster WG, Nazarewicz RR, Bikineyeva AT, Harrison DG, Dikalov SI. Mitochondrial Cyclophilin D in Vascular Oxidative Stress and Hypertension. Hypertension. 2016 Apr 11.
  5. Dikalov SI, Nazarewicz RR*, Bikineyeva A, Hilenski L, Lassègue B, Griendling KK, Harrison DG, Dikalova AE. Nox2-Induced Production of Mitochondrial Superoxide in Angiotensin II-Mediated Endothelial Oxidative Stress and Hypertension. Antioxid Redox Signal. 2014 Jan 10;20( 2):281-94.
  6. Nazarewicz RR, Dikalova A, Bikineyeva A, Dikalov SI. Nox2 as a potential target of mitochondrial superoxide and its role in endothelial oxidative stress. Am J Physiol Heart and Circ. 2013 Oct 15;305 (8):H1131-40.
  7. Nazarewicz RR*, Dikalov SI. Mitochondrial ROS in the pro-hypertensive immune response. Am J Physiol Regul Integr Comp Physiol. 2013 Jul 15;305 (2):R98-100.
  8. Nazarewicz RR, Dikalova A, Bikineyeva A, Ivanov S, Kirilyuk IA, Grigor'ev IA, Dikalov SI. Does Scavenging of Mitochondrial Superoxide Attenuate Cancer Prosurvival Signaling Pathways? Antioxid Redox Signal. 2013 Aug 1;19 (4):344-9.
  9. May JM, Qu ZC, Nazarewicz R, Dikalov S. Ascorbic acid efficiently enhances neuronal synthesis of norepinephrine from dopamine. Brain Res Bull. 2013 Jan;90:35-42.
  10. Nazarewicz RR, Bikineyeva A, Dikalov SI. Rapid and specific measurements of superoxide using fluorescence spectroscopy. J Biomol Screen. 2013 Apr;18(4):498-503.
  11. Dikalov SI, Nazarewicz RR. Angiotensin II-Induced Production of Mitochondrial Reactive Oxygen Species: Potential Mechanisms and Relevance for Cardiovascular Disease. Antioxid Redox Signal. 2013 Oct 1;19 (10):1085-94.
  12. Triantafyllou A, Bikineyeva A, Dikalova A, Nazarewicz R, Lerakis S, Dikalov S. Anti-inflammatory activity of Chios mastic gum is associated with inhibition of TNF-alpha induced oxidative stress. Nutr J. 2011 Jun 6;10:64.
  13. Nazarewicz RR, Salazar G, Patrushev N, San Martin A, Hilenski L, Xiong S, Alexander RW. Early endosomal antigen 1 (EEA1) is an obligate scaffold for angiotensin II-induced, PKC-alpha-dependent Akt activation in endosomes. J Biol Chem. 2011, 28;286 (4):2886-95.
  14. Dikalova AE, Bikineyeva AT, Budzyn K, Nazarewicz RR, Lewis W, Harrison DG and Dikalov SI. Therapeutic targeting of mitochondrial superoxide in hypertension. Circ Res 2010 Jul 9;107 (1):106-16.
  15. Parihar A, Parihar MS, Nazarewicz R, Ghafourifar P. Importance of cytochrome c redox state for ceramide-induced apoptosis of human mammary adenocarcinoma cells. Biochim Biophys Acta 2010 Jul;1800 (7):646-54.
  16. Xiong S, Salazar G, San Martin A, Ahmad M, Patrushev N, Helenski L, Nazarewicz RR, Ma M, Fukai MU, Alexander RW. PGC-1alpha Serine 570 Phosphorylation and GCN5-Mediated acetylation by Angiotensin II Drive Catalase Downregulation and Vascular Hypertrophy. J Biol Chem. 2010 Jan 22;285 (4):2474-87.
  17. Parihar MS, Parihar A, Chen Z, Nazarewicz R, Ghafourifar P. mAtNOS1 regulates mitochondrial functions and apoptosis of human neuroblastoma cells. Biochim Biophys Acta 2008 Jun;1780 ( 6):921-6. 
  18. Parihar MS, Nazarewicz RR, Kincaid E, Bringold U, Ghafourifar P. Association of mitochondrial nitric oxide synthase activity with respiratory chain complex I. Biochem Biophys Res Commun 2008 Feb 1;366 (1):23-8. 
  19. Ghafourifar P, Mousavizadeh K, Parihar MS, Nazarewicz RR, Parihar A, Zenebe WJ. Mitochondria in multiple sclerosis. Front Biosci 2008 Jan 1; 13:3116-26.
  20. Nazarewicz RR, Zenebe WJ, Parihar A, Parihar MS, Vaccaro M, Rink C, Sen CK, Ghafourifar P. 12(S)-hydroperoxyeicosatetraenoic acid (12-HETE) increases mitochondrial nitric oxide by increasing intramitochondrial calcium. Arch Biochem Biophys 2007 Dec 1; 468 (1):114-20.
  21. Nazarewicz RR, Ziolkowski W, Vaccaro PS, Ghafourifar P. Effect of short-term ketogenic diet on redox status of human blood. Rejuvenation Res 2007 Dec;10 (4):435-40.
  22. Zenebe WJ, Nazarewicz RR, Parihar MS, Ghafourifar P. Hypoxia/reoxygenation of isolated rat heart mitochondria causes cytochrome c release and oxidative stress; evidence for involvement of mitochondrial nitric oxide synthase. J Mol Cell Cardiol 2007 Oct;43 (4):411-9.
  23. Nazarewicz RR, Zenebe WJ, Parihar A, Larson SK, Alidema E, Choi J, Ghafourifar P. Tamoxifen induces oxidative stress and mitochondrial apoptosis via stimulating mitochondrial nitric oxide synthase. Cancer Res 2007 Feb 1;67 (3):1282-90.

Books and book chapters

  1. Dikalov S, Nazarewicz R. Measurements of reactive oxygen species in cardiovascular studies. In: Systems Biology of Oxidative Stress.  Springer Science, 2014   
  2. Ghafourifar P, Parihar MS, Nazarewicz R, Zenebe WJ, Parihar A. Detection assays for determination of mitochondrial nitric oxide synthase activity; advantages and limitations. Methods Enzymol 2008;440:317-34
  3. Babicz–Zielińska E., Nazarewicz R., Rybowska A. Dietary treatment in disease. AM Gdynia 2005
  4. Nazarewicz R "Physical activity, nutrition state and oxidative stress in young sportsmen". Warsaw Agricultural University, Faculty of Human Nutrition and Consumer Sciences, Warsaw 2003
  5. Babicz–Zielińska E., Nazarewicz R. Basic knowledge of nutrition. AM Gdynia 2003

Abstracts

  1. Nazarewicz RR, Dikalova A, Fessel JP, Itani H, McMaster W, Bikineyeva A, Harrison DG, Dikalov S. Cytokine-angiotensin II Interplay in Cyclophilin D-mediated Vascular Oxidative Stress and Hypertension. Hypertension 2014, High Blood Pressure, San Francisco, CA 2014, peer reviewed
  2. Nazarewicz RR, Dikalova A, Fessel JP, Itani H, McMaster W, Harrison DG, Dikalov S. Sirt3 Impairment and SOD2 Hyperacetylation Drive Vascular Oxidative Stress and Hypertension. Hypertension 2014, High Blood Pressure, San Francisco, CA 2014, peer reviewed
  3. Nazarewicz RR, Saleh MA, Wu J, Dikalov S, Harrison DG. Mitochondrial hydrogen peroxide in T cell activation.  Hypertension 2014, High Blood Pressure, San Francisco, CA 2014, peer reviewed
  4. Nazarewicz RR, Dikalova A, Bikineyeva A, Egnatchik R, Young J, Harrison DG, Dikalov SI. T cell mitochondrial ROS in development of hypertension. Experimental Biology Meeting, San Diego, California, 2014 peer reviewed 
  5. Nazarewicz RR, Dikalova A, Bikineyeva A, Harrison DG, Dikalov SI. Mitochondrial ROS in the pro-hypertensive immune response. Experimental Biology Meeting, Boston, MA, 2013 peer reviewed
  6. Nazarewicz R, Bikineyeva A, Harrison DG, Dikalov S. Rapid and Specific Measurements of Superoxide Using Fluorescence Spectroscopy. Experimental Biology Meeting, San Diego, California, 2012 peer reviewed
  7. Nazarewicz R, Dikalova A, Bikineyeva A, Hilenski L, Mayorov V, Lassègue B, Harrison D, Griendling K, Dikalov S. Mechanism regulating cytoplasmic superoxide production by mitochondria. Experimental Biology Meeting, Washington, 2011.
  8. Nazarewicz R, Dikalova A, Bikineyeva A, Hilenski L, Lassègue B, Harrison D, Griendling K, Dikalov S. Mitochondrial ATP-Sensitive Potassium Channel Regulates Cytoplasmic NADPH Oxidase Activity through Mitochondrial Superoxide. SFRBM Annual Meeting, Orlando 2010, Free Rad Biol Med 2010, 20.
  9. Dikalov S, Dikalova A, Nazarewicz R, Bikineyeva A, Mayorov V, Hilenski L, Lassègue B, Harrison D, Griendling K. Role of Nox2-induced Reverse Electron Transfer in Production of Mitochondrial Reactive Oxygen Species, Endothelial Dysfunction and Hypertension. SFRBM Annual Meeting, Orlando 2010, Free Rad Biol Med 2010, 27.
  10. Nazarewicz RR, Salazar G, Patrushev N, Martin AS, Hilenski L, Xiong S, Ushio-Fukai M, Alexander RW. Central role of EEA1 in organization of Angiotensin II signaling leading to Akt activation in early endosomes. American Heart Association Scientific Meeting, Orlando, USA 2009, Circulation 2009, Supp.
  11. Dikalova AE, Bikineyeva AT, Budzyn K, Nazarewicz RR, Lewis W, Harrison DG and Dikalov SI Therapeutic targeting of mitochondrial superoxide in hypertension. 16th Annual Meeting of Society for Free Radical Biology and Medicine, USA 2009, Free Rad. Biol Med 2009, 46, Supp.1. 
  12. Dikalova AE, Bikineyeva AT, Budzyn K, Nazarewicz RR, Lewis W, Harrison DG and Dikalov SI. Therapeutic targeting of mitochondrial superoxide in hypertension. American Heart Association Scientific Meeting, Orlando, USA 2009,Circulation 2009.
  13. Dikalova AE, Bikineyeva AT, Budzyn K, Nazarewicz RR, Lewis W, Harrison DG and Dikalov SI. Role of mitochondrial superoxide in endothelial dysfunction and hypertension. 6th  International Conference Reactive Oxygen Species, Nitric Oxide, Antioxidants, and Human Health, Smolensk 2009, Russia (monograph).
  14. Nazarewicz RR; Patrushev NA; Hilenski L; Ushio-Fukai M; Alexander RW. Novel Role of Early Endosome Antigen 1 in Reactive Oxygen Species-dependent AT1 Receptor-mediated Signaling Linked to Hypertrophy in Vascular Smooth Muscle Cells. American Heart Association Scientific Meeting, New Orleans, USA 2008, Circulation 2008;118(18):S313-S314. 
  15. Ghafourifar P, Nazarewicz R, Zenebe WJ, Chen Z, Parihar MS, Parihar A. Heart mitochondria nitric oxide synthase 2007; Mitochondrial Medicine Meeting, San Diego, USA 2007, Mitochondrion 2007;7(6): 404.
  16. Nazarewicz R., Ziólkowski W. Effect of high fat diet on oxidative stress in human subjects. 4th International Conference, Lublin, Poland 2006.
  17. Nazarewicz R. Uric acid and oxidative stress in young sportsmen. International Conference Phisiological conditions of diet application. Warsaw, Poland 2004, (monograph 354-359).
  18. Nazarewicz R., Babicz-Zielińska E. Body composition related to physical activity and protein intake. 9 th European Nutrition Conference, Rome, Italy 2003.
  19. Nazarewicz R., Babicz-Zielińska E. Protein intake and oxidative stress among youth of different physical activity. 9 th European Nutrition Conference, Rome Italy 2003. 


PROFESSIONAL ACTIVITIES                                                              

Intramural

  • 2011-2013 Conducting trainings for students, faculty and stuff at the Vanderbilt University Free Radicals in Biology and Medicine Core lab 
  • 2011-2013 Developing methods for the detection of reactive oxygen species, Vanderbilt 
  • University Free Radicals in Biology and Medicine Core lab 
  • 2013 Vanderbilt Vascular Biology Seminar- “Measurements of nitric oxide and reactive oxygen species in vasculature”
  • 2013 Judging poster session during VUMC Postdoctoral Research and Shared Resources Symposium 
  • 2012 Lecture and seminar during 2012 Vasculatata workshop on vascular biology 
  • 2006-2007 Supervision of undergraduate students; The Ohio State University, Laboratory of mitochondrial biology, Columbus, OH
  • 2005-2006 Organization of conferences; National Meetings of Students Associations,  Poland
  • 2004-2006 Member of student recruitment board at Gdynia Maritime University, Poland
  • 2002-2007 Designing and mentoring research projects for graduate and undergraduate students at Gdynia Maritime University, Poland and The Ohio State University, Columbus, OH

Extramural

  • ad hoc reviewer Antioxidants and Redox Signaling, Circulation Research, The International Journal of Biochemistry & Cell Biology  

Honors and awards

  • 2006 Travel award to the Ohio State University
  • 2005 Rector of Gdynia Maritime University Young Scientist Award, Poland
  • 2002 Rector of Gdynia Maritime University Young Scientist Award, Poland

TEACHING ACTIVITIES:

  • 2011-2013 J. Sniadecki University School of Physical Education, Poland 
  • Summer undergraduate courses: Sports nutrition and metabolism
  • 2003-2006 Gdynia Maritime University, Poland
  • Under- and graduate courses: Human nutrition and metabolism, Physiology and anatomy in nutrition, Assessment of nutrition status 
  • 2003-2006 J. Sniadecki University School of Physical Education, Poland
  • Undergraduate courses: Sports nutrition, Human nutrition and metabolism, Physiology and anatomy in nutrition
  • 1997-2006 Gdynia Maritime University, Poland
  • Undergraduate courses: Human nutrition and metabolism, Physiology and nutrition
  • 1995-1997 J. Sniadecki University School of Physical Education, Poland
  • Undergraduate courses: Biochemistry, Bioenergetics